DNA
RS2

Part:BBa_K743001:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)

Synechocystis PCC6803 neutral recombination site.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
    Illegal XhoI site found at 629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.

This part has a EcoR1 site at position 132 wich wasn´t mutated so that there´s complete homology with Synechocystis chromosome region. The restricion site should´t be a problem for standard assemblies as long as this part is used as a downstream brick.

Source

PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337

References